7th ISF
CHEM038 - Development of Glucose Oxidase (GOx)-Based Biosensors with Enzyme Covalently Bonded to a Gelatin/Zein/Prussian Blue Sensor Surface Coating
Glucose is an essential substance in the human body, but its excessive presence can lead to diabetes. A cost-effective and valuable tool for non-invasive glucose monitoring in diabetes management without the use of carbon nanotubes draws inspiration from previously published works: Soysa et al., 2021 demonstrate the synergistic effect of immobilizing gelatin-GOx and utilizing zein as a molecular filter for glucose detection, leading to excellent glucose analytical quality testing, and Rattanopas et al., 2022 demonstrate successful production and characterization of a sandwich-type amperometric sensor for detecting cathodic hydrogen peroxide, comprising a H₂O₂-signalling Prussian blue/carbon nanotube (CNT) layer protected by a gelatin/zein glaze to prevent PB leakage. By bringing together the advantages of previous published works, we aim to develop a screen-printed carbon electrode (SPCE) coated with sustainable materials: Prussian Blue, zein, and gelatin immobilized by the glucose oxidase enzyme. This was performed by immobilizing the glucose oxidase enzyme on the SPCE using the drop and dry technique, with PB beneath, zein in the middle, and gelatin on top. The results show that a newly modified biosensor has a sensitivity value of 0.319 ± 0.00509 nA µM⁻¹ (R² ≥ 0.995) in detecting glucose solutions with high reproducibility and the detection limit of 10 μM and is interference-free from uric acid and ascorbic acid, two potentially interfering substances. The developed biosensor has the potential to ultimately improve the quality of life for individuals living with diabetes.
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