7th ISF

BIOL038 - Glucose Recovery From Plant-Based Wastes by Cellulolytic Bacteria Found in Mega-Herbivore Feces

In this study, we aim to enhance the value of plant-based waste beyond conventional composting methods. Specifically, we focus on glucose recovery from cellulose present in plant-based waste, employing cellulolytic bacteria isolated from Banteng (Bos javanicus) and elephants (Elephas maximus), identified as Bio Safety Level 1 microorganisms. The bacteria collected from Banteng are labeled as BTS, while those from elephants are labeled as ELS. Our cellulolytic activity tests, utilizing the clear zone: colony zone ratio on CMC agar plates and Congo red straining, reveal that BTS01 exhibits the highest average ratio, followed by ELS06, ELS07, and Bacillus subtilis, respectively. Subsequent experiments employing tissue paper as a substrate demonstrate that BTS01 produces the highest amount of glucose, as measured by a spectrophotometer, surpassing the other three bacteria. Additionally, cumulative glucose production over time using bagasse is recorded. Notably, when utilizing coco husk as a substrate, ELS06 and ELS01 exhibit superior glucose recovery compared to BTS01. This finding suggests the potential versatility of cellulolytic bacteria in glucose extraction from different plant-based waste materials.

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