7th ISF

BIOL035 - Determination of the Optimal Condition for Caladium bicolor Growth

Caladium bicolor is an ornamental plant in global plant market and holds significant commercial importance. This project seeks to optimize the condition for callus induction in Caladium bicolor using in vitro culture technique. In the preliminary experiment, the leaf explants underwent surface sterilization using different sodium hypochlorite concentration. 4% v/v sodium hypochlorite had proven to provide the highest viability rate, of 84% (5% bacterial contamination). Subsequently, the leaf explants were cultured in semi-solid and liquid Murashige and Skoog (MS) media with varying concentrations of cytokinin, BA (0 and 2 mg/L) and auxin, IAA (0 and 0.5 mg/L). The results showed that semi-solid media were more effective in inducing callus, shoot, and root formation, whereas liquid media only facilitated the enlargement of the explants. The semi-solid MS media supplemented with 2 mg/L BA and 0.5 mg/L IAA was found to be the most efficient media for inducing callus, shoot, and root formation, reaching up to 20% within day 30 and 100% in day 42. These findings not only contribute to the optimization of in vitro culture techniques for Caladium bicolor but also underscore the potential for enhanced commercial cultivation practices.

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